EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. Avantor®, a Fortune 500 company, is a leading global provider of mission-critical products and services to customers in the biopharma, healthcare, education & government, and advanced technologies & applied materials industries. It features a modular design that allows users to add or change detection cartridges according to their needs. Avantor®, a Fortune 500 company, is a leading global provider of mission-critical products and services to customers in the biopharma, healthcare, education & government, and advanced technologies & applied materials industries. Products : SpectraMax i3x reader, SpectraMax iD5 reader, SpectraMax iD3 reader, General, FilterMax F5 reader, SpectraMax ABS reader, SpectraMax L reader, SpectraMax M5/M5e reader This page contains the table of contents of resources available for the SpectraMax Microplate Reader Suite of Products. Get more information about Fluorescence Polarization SpectraMax Detection Cartridges, which offer great sensitivity for SpectraMax i3x and Paradigm Systems. It allows you to explore cellular . The SpectraMax® i Series Multi-Mode Mode Microplate Readers offer the ideal solution for any application or budget. The reader can be equipped with a NanoBRET detection for a 4-fold improvement in limit of. The Relative Luminescence Units (RLU) were measured using the SpectraMax i3x Luminescence Glow cartridge (Lum 384) (0200-7015POS, Molecular Devices) and normalized to the cell count or area, which were determined using the Mini MaxTM 300 Imaging Cytometer (5024062, Molecular Devices). Multimode Reader Comparison Chart. The SoftMax Pro Software provides integrated instrument control. Fluorescence at 365 nm excitation and 450 nm emission filters was determined based on a microplate fluorometer (SpectraMax i3x, Molecular Devices, Beckman Coulter, CA, USA). SoftMax Pro GxP software is a special release that includ es full user and permission control and audit trail generation in addition to all the dataThe SpectraMax i3x reader and MiniMax cytometer can be used with FluoroBlok cell culture inserts to directly count cells from high-quality images to measure cellular migration over time. Located at the right front of the SpectraMax Plus 384, the cuvette chamber has a lid that lifts up, allowing you to insert or remove a cuvette. Spectral Fusion Illumination is a powerful combination of Xenon flash lamp and LEDs that provides unmatched signal strengthSpectraMaxABSInstrumentInstallationGuide 4 5065391A ComputerIntegration EachMolecularDevicesmicroplatereaderisshippedwithalicensekeyfortheSoftMax®ProSpectraMax iD5 reader. XPS spectra were obtained from PHI Versaprobe II. The reader can be equipped with a NanoBRET detection for a 4-fold improvement in limit of. The MiniMax cytometer with SoftMax® Pro Software can be used to image and analyze a variety of cells. The SpectraMax® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of modular upgrades for Western Blot, Imaging, and Fast Kinetics with Injectors. The SpectraMax® i3 Platform's base system features an integrated optical system enabling top and bottom reads for 6-, 384- well microplates and launches with three broad detection modes: luminescence, absorbance, and fluorescence. Average 98 stars, based on 1 article reviews. Products : SpectraMax i3x reader, SpectraMax iD5 reader, SpectraMax iD3 reader, General, FilterMax F5 reader, SpectraMax ABS reader, SpectraMax L reader, SpectraMax M5/M5e reader This page contains the table of contents of resources available for the SpectraMax Microplate Reader Suite of Products. SpectraMaxi3xMulti-ModeDetectionPlatformUserGuide 72 5039228D. Auto PMT Challenge: A standard Photomultiplier Tube (PMT) or detector limits the dynamic range of your instrument. 303 unable to cal dark current. For more detailed information and technical support of Molecular Devices instruments or software,The SpectraMax® Paradigm Multi- Mode Microplate Reader with Tunable Wavelength (TUNE) Detection Cartridge from Molecular Devices combines the flexibility of wavelength scanning with the high sensitivity of filters. ZERO BIAS - scores, article reviews, protocol conditions and more. 2 in. Microplate optimization should be performed the first time the assay is run, and subsequently for . SpectraMax i3x, onboard luminescence 121. As per Volza’s India Import data, Spectramax import shipments in India stood at 365, imported by 145 India Importers from 28 Suppliers. 306 plate air cal. Controls containing all reaction components with either DI water or a fluorescent standard in place of the fluorescent substrate were run alongside the experimental samples. The possibility of user upgrading the system with an application cartridge and an imaging cytometer makes this device the most flexible microplate. Capture Flash Assays with Ease. Patented user-exchangeable cartridge design expands the system's detection capability making it. Spectral Fusion Illumination is a powerful combination of Xenon flash lamp and LEDs that provides unmatched signal strengthThe SpectraMax i3x reader grows with you as your research areas expand. For wavelengths in the UV range above 220 nm,. Animal imaging assay was performed on a IVIS R Lumina Series III small animal optical system. , 2011). 3: The cuvette chamber. UV-Vis absorbance, fluorescence and luminescence • Accommodates plate types from six to 384-well format • Easily upgradeable with interchangeable filter cubesSpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. View online or download 1 Manuals for Molecular Devices SpectraMax i3x. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. Table 1. Eliminate time-dependent substrate addition steps. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 5 1. An MPO assay has been performed to quantify the inflammation reduced by the GAR-PLGA-ES100 NPs in the inflamed cells . It allows the user to determine the total concentration of protein in a solution. Additionally, user-installable options allow the SpectraMax i3 System to grow with your changing application needs to fulfill and go beyond the standard reader applications, protecting your initial investment. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. Supplier Page. The cell imaging was captured on Zeiss LSM 980 confocal microscopy. Absorbance was measured on the SpectraMax i3x Multi-Mode Platform at 475 nm and 660 nm after 1-4 hours of incubation. Ideal for a wide range of applications, including Western Blot analysis, this SpectraMax i3x includes the additional MiniMax 3000 Imaging Cytometer module for advanced cell imaging. 315 can't find zero order. detection cartridge LUM96: 59. The fluorescence quantum yield (QY) of the Pdots was measured by a UV−NIR absolute PL QY. Explore more about the SpectraMax i3x Multi-Mode Plate Reader, a user-upgradeable detection modules allow you to expand the capabilities of your microplate reader when your research requires it. Measured using the SpectraMax DuoLuc Reporter Assay Kit on the SpectraMax i3x microplate reader. The SpectraMax i3x microplate reader measures absorbance, luminescence and fluorescence as standard, and provides the flexibility to add novel detection capabilities without the need for service. Explore a wealth of applications in one future-ready system that measures spectral-based absorbance, fluorescence, and luminescence. Turbidity measurements were performed with a Spectra Max® i3x microplate reader to study the effect of oxidation on the kinetics of sFN formation. SpectraMax® i3x Multi-Mode Detection Platform, Molecular Devices. The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget-friendly solution with three modes of detection for UV-Vis absorbance, fluorescence, and luminescence. SpectraMax i3x Multi-Mode Detection Platform User Guide. The SpectraMax i3x Injector Cartridge with SmartInject Technology is a data sheet that describes the features and benefits of this innovative accessory for the SpectraMax i3x Multi-Mode Detection Platform. Plate chamber design of the SpectraMax category 1 microplate readers. Using the SpectraMax i3x reader, the preconfigured SpectraMax Glo Steady-Luc Reporter assay protocol in SoftMax Pro Software was used to measure luminescence. Description 1. SpectraMax i3x 多功能微孔板读板机 SpectraMax Paradigm 多功能微孔板读板机 SpectraMax M5e 多功能微孔板读板机 扫一扫关注我 们的官方微信 Molecular Devices 大中华区 全国咨询服务热线: 400-820-3586 Email: info. Microplate readers, multi-mode, SpectraMax® i3x. each new assay. Detection of pro-inflammatory factors. The CellTiter-Glo assay from Promega uses luciferase. The plate was covered and incubated for one hour at room temperature. To read HTRF®, the SpectraMax i3x® must be first equipped with the SpectraMax i3x® Cisbio HTRF® cartridge, which enables the simultaneous measurement of both 620nm donor and 520 nm acceptor emissions. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. Louis, MO) and used as received without further purification. com The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. The SpectraMax MiniMax 300 Imaging Cytometer option can be added at any time to the SpectraMax i3x System, providing an expandable platform that evolves with your changing needs. Because a PMT needs to be set to a specified gain to reach a specific sensitivity,SpectraMax Paradigm Microplate Reader is a versatile and customizable device that can perform a wide range of detection modes, such as absorbance, fluorescence, luminescence, time-resolved fluorescence, and AlphaScreen. This application note describes the use of the SPECTRAmax ® GEMINI XS from Molecular Devices to study the performance of the LIVE/DEAD ® Viability/Cytoxicity Assay Kit for animal cells. 2 GxP Software is the latest, most secure software to achieve full FDA 21 CFR Part 11 and EudraLex Annex 11 compliance with. 58% for the detection module and 2. The SpectraMax i3x Multi-mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with added functaionlity of modular upgrades for Western Blot, Imaging, and Fast Kinetics with Injectors. Formicro-volumemeasurements,theinstrumentsupportsSpectraDrop24-wellSpectraMax i3 Install Guide; SpectraMax i3; SpectraMax i3x; SpectraMax iD3; SpectraMax iD5; SpectraMax Mini; SpectraMax Mini Install Guide; SpectraMax Mini Reader Plug-In Download Page (required for SoftMax Pro 7. All test measurements and calculations are handlediD5. Immunofluorescence StainingAfter cooled down to room temperature, the concentrations of the solution were measured by microplate reader (Spectra Max i3x) at 490 nm (Kagi and Schaffer, 1988). Example: [Drawer]. The SpectraMax® 190 spectrophotometer is ideal for most life science applications, especially DNA analysis. In SoftMax® Pro, the following Automix settings are available for the SpectraMax i3/i3x Instrument: Intensity : Low, Medium, or High. Recommendations for SpectraMax i3/i3x plate readers The Molecular Devices SpectraMax i3/i3x allows use of the pH-Xtra Glycolysis Assay with time-resolved fluorescence (TRF) detection. , 2021). Controls containing all reaction components with either DI . techsupport. GFP expression imaged on the cytometer. Controls containing all reaction components with either DI water or a fluorescent standard in place of the fluorescent substrate were run alongside the experimental samples. Relative mitochondrial polarization was expressed as the ratio of emission at 590 nm to that at 530 nm (Shin et al. ZERO BIAS - scores, article reviews, protocol conditions and moreSpectraMax i3x offers all the techniques you need to explore cell pathway in a single system. Devices instruments including: SpectraMax®, VersaMax, VMax®, EMax®, Gemini, ThermoMax, and FlexStation® readers. Prime function 20 amol. Get a front row view of your cells. 1. AlphaScreen and AlphaLisa detection using a 680 nm excitation laser diode and a 570/100 nm emission filter. **On the SpectraMax iD3/iD5 readers only All graphs are for illustrative purposes only. ; India imports most of its Spectramax from United States, China and Austria and is the largest importer of Spectramax in the World. Promega. For more detailed information and technical support of Life Technologies assays, please call 1-800-955-6288 and enter extension 40266 or email drugdiscoverytech@lifetech. Prepare your i3/i3x instrument for shipment. The calculated LOQ values were 0. 1. 2 in. . SpectraMax i3x and Paradigm readers require the FP-FLUO. 0. The SpectraMax® i3x Multi-Mode Microplate Reader with the SpectraMax® MiniMax™ 300 Imaging Cytometer is equipped with transmitted light (TL), green fluorescence (Ex/Em: 460/541), and red (625/713) fluorescence channels. The SpectraMax i3x multi-functional detection platform launched by Molecular Devices can meet the multi-functional detection system with unlimited possible applications as your future needs change. If desired, a high-range standard curve may be prepared from 1 ng/mL to 1 μg/mL, or a low-range standard curve may be prepared from 25 pg/mL to 25 ng/mL. Figure 4. INTRODUCTION The SpectraMax M2 and SpectraMax M2e Multidetection Readers are monochromator based microplate readers that have 6well to 38 4well microplate reading capability as wellSpectraMax i3x The SpectraMax i3x from Molecular Devices is a multi-mode detection system that evolves with your future needs and offers an unlimited breadth of application possibilities. 2. The SpectraMax® i3 Multi-Mode Detection Platform comes with standard spectral absorbance, fluorescence, and luminescence detection. Fluorescence emission spectra were collected on an ELISA molecular system (Spectra Max i3x). SoftMax Pro GxP software is a special release that includ es full user and permission control and audit trail generation in addition to all the dataBased on the SpectraMax i3x system, ACROBiosystems provides biomedical research and development services for customers seeking quantification and detection of antibodies-antigen interaction. The SpectraMax i3x reader allows you to unravel the mysteries of science by exploring cellular pathways and protein activation and expression in one system. Quick imaging and analysis of cells gives you a front-row view of the phenotypic changes that accompany. Faster data acquisition and analysis with preconfigured SoftMax Pro protocols. The chamber. SpectraDrop Abs Protein Quant (i3-i3x)Molecular Devices SpectraMax i3x Multi-Mode Detection Platform. The Cuvette Chamber (SpectraMax Plus 384 only) Figure 1. pathways and protein activation and expression in one system. Published Dec 16, 2020 | Updated Dec 16, 2020. , 2011). The SpectraMax i3x reader is engineered to perform with Spectral Fusion Illumination for increased sensitivity across the entire wavelength range, and a cooled photomultiplier tube (PMT) for improved detection in extremely low light. It allows you to explore cellular pathways and protein activation and expression in one system. This UV-visible absorbance microplate reader provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with the only temperature-independent method for pathlength correction. Features technology from the SpectraMax i3x and MiniMax 300 Imaging Cytometer. 2 only) SpectraMax M-series Microplate readers. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. The SpectraMax ® i3x reader provides the. These robust readers have been placed in labs from Antarctica to the. Quantification of NETs was performed with the SpectraMax i3x microplate-reader (Molecular Devices, United Kingdom). In this application note, we describe how the SpectraMax ® i3x Multi-Mode Microplate Reader with SpectraMax Injector Module is used to perform calcium assays on two different cell lines. 2 x 10-14. It allows you to explore cellular . Located at the right front of the SpectraMax Plus 384, the cuvette chamber has a lid that lifts up, allowing you to insert or remove a cuvette. Download PDF. It allows you to explore cellular pathways and protein activation and expression in one system. Eliminate time-dependent substrate addition steps. The SpectraMax i3x reader allows you to unravel the mysteries of science by exploring SpectraMax i3 Install Guide; SpectraMax i3; SpectraMax i3x; SpectraMax iD3; SpectraMax iD5; SpectraMax Mini; SpectraMax Mini Install Guide; SpectraMax Mini Reader Plug-In Download Page (required for SoftMax Pro 7. SpectraMax i3x microplate reader and SoftMax Pro Software protocol. Lower limits of detection for ATP determined using the ATPlite . However, no piece of equipment is immune to breaking down. As. **On the SpectraMax iD3/iD5 readers only All graphs are for illustrative purposes only. Spectral Fusion Illumination is a powerful combination of Xenon flash lamp and LEDs that provides unmatched signal strength and superior sensitivity across the full. Pick the best speed, sensitivity, and price for your needs. User-upgradeable detection modules allow you to expand the capabilities of your microplate reader when your research requires it. CRITICAL STEP Avoid air bubbles; reverse pipetting the luciferase reagent into the plate is a good way to achieve this. 0 mm hex key. The turbidity. 3. The LDH activity in the cultural media was measured based on the reaction between LDH and lactic acid, which led to the generation of pyruvic acid, and pyruvic acid could become brown in alkaline environments with 2,4. Benefits • User-upgradeable application modules including cellular imaging • Sensitivity across spectrum with Spectral Fusion™ Illumination • Expanded dynamic range with cooled PMT • Control and analytics provided by SoftMax® Pro Software SpectraMax i3x The SpectraMax i3x from Molecular Devices is a multi-mode detection system that evolves with your future needs and offers an. Multi-Mode Reader. at 540 nm using a Spectra Max i3x microplate reader (Molecular devices, San Jose, CA). Fluorescent Polarization: Solid black plates, as above. HTRF is a proprietary time-resolved fluoresce nce technology that overcomes This assay workflow is easily performed using the SpectraMax i3x Multi-Mode Microplate Reader with SpectraMax Injector Cartridge, which is fully DL Ready validated. (SpectraMax i3/i3x) CatchPoint SimpleStep ELISA (SpectraMax Paradigm) SpectraDrop. For aqueous solutions, our patented* PathCheck® sensor technology senses the depth of the liquid in each microplate well, and normalizes the absorbance value to a 1-cm pathlength. See Unpacking the SpectraMax Paradigm Instrument on page 1. . SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 5 1. The Molecular Devices SpectraMax i3X Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. 314 low lamp level warning. In this case, the SpectraMax i3x reader works as a flash-lamp based microplate reader as described in the supporting application note “The AutoPMT™ feature for an extended dynamic range in the SpectraMax iD and M series Multi-Mode Readers”. 価格、見積もり、お近くの販売店を. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire. 200-800 nm using Spectra Max i3X microplate reader. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. The standard solutions (0. spectramax i3x (Danaher Inc)The SpectraMax® i3x multi-mode microplate reader is a configurable, versatile microplate reader that evolves with your future needs and allows you to investigate every aspect of a cellular pathway. SpectraMax i3x plate reader (Molecular Device, contact supplier directly) Vacuum pump system (e. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. KEY FEATURES • Combine cellular imaging and microplate assays into a workflow • Eliminate cell staining for cell counting and confluency with StainFree Technology*On the SpectraMax i3x reader only. The user guide also covers the features and specifications of the SpectraMax MiniMax 300. Increased fluorescence performance. Because a PMT needs to be set to a specified gain to reach a specific sensitivity,*On the SpectraMax i3x reader only. The PMT gain can be set to manual, high, medium, low, or automatic (Figure 3A). Accessories for SpectraMax® i3x Multi-Mode Detection Platform, Molecular Devices. Assay setup for a 384-well low-volume plate. Learn how you can change the detection module of your SpectraMax i3x instrument in just a few seconds. Supplier: Molecular Devices. SpectraMax® iD3/iD5. com. 1) Select the correct plate and the wells that you want to measure. Learn about the SpectraMax i3x Multi-Mode Plate Reader capabilities and options in this short video. Reaction catalyzed by the firefly luciferase. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. Then, the cell area was calculated for each. 0´ 104 1. Explore a wealth of applications with the future-ready SpectraMax i3x. 660-665 Eskdale Road. Microplate Readers SpectraMax. Molecular Devices Corporation. 12911151) 0. Equipped with transmitted light (TL), green (Ex/Em: 460/541) and red (625/713) fluorescence channels, the MiniMax cytometer with SoftMax® Pro Software can be used to image and. 1 1 10 100 e e e e e e eThe SpectraMax MiniMax 300 Imaging Cytometer option can be added at any time to the SpectraMax i3x System, providing an expandable platform that evolves with your changing needs. Fluorescence Microplate Reader Comparison Chart. . Molecular Devices SpectraMax ® i3x MultiMode Microplate Reader, in combination with luminescent assays for cell viability and cytotoxicity, provides a sensitive and rapid way to measure the number of viable cells in culture and quantify the cytotoxic effects of experimental treatment. ，相关视频：多功能酶标仪SpectraMax i3x的维护操作-注射器卡盒，多功能酶标仪SpectraMax iD5的维护操作-注射器卡盒，多功能酶标仪SpectraMax i3x的维护操作-卡盒的安装和拆卸，细胞计数及活率分析仪 Vi-CELL BLU 功能&操作讲解，CRISPR基因编辑系统介绍：Cas9和Cas12a，流式. Settings not required by a reader are indicated by ‘---’. Recommended settings for SpectraMax iD5 and i3x readers. Easily upgrade or switch between detection modes with user-exchangeable modules – just open the drawer and insert the module you want. Description 1. SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. This technical note demonstrates validation of the SpectraMax® i3x Multi-Mode Microplate Reader for NanoBRET™ assays. 58% for the detection module and 2. flValitaTiter (i3x i3) ValitaTiter (iD5) ValitaTiter (M5 M5e Flex3) ValitaTiter (Paradigm) ValitaTiter (FilterMax F5) Assay-Specific. More Product Information. 0´ 104 4. For 40 years, we have partnered with scientists to help them achieve landmark discoveries. Manufacturers try to design their instruments so they can be. china@moldev. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user-configurable. 4. SpectraMax i3x读板机针对96孔板和384孔板进行检测时，最低检测下限可至10细胞每孔。 此种检测方法的灵敏度远远高于标准比色法和绝大多数荧光法。 CellTiter-Glo试剂检测线性范围覆盖所有细胞浓度区间，动态学检测范围大于3个数量级且r2 > 0. i3x. Ease of use. The SpectraMax i3x measures monochromator-based absorbance, fluorescence and luminescence with a future-ready platform that can evolve with the researcher’s needs. Generate more data points without the need to dilute. The SpectraMax i3x reader grows with you as you continue to advance your research, enabling you to unravel the mysteries of science by exploring cellular pathways and protein activation and expression in one system. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 5 1. 01 0. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. About the reader setup recommendations The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. The NET-specificity of the detected signal was determined by incubation with DNase I (50 U/mL, Qiagen, Germany). See SpectraMax® Microplate Reader User Manual Download Page. 在SpectraMax i3x和SpectraMax iD5微孔板读板机上使用 SoftMax Pro软件绘制标准曲线，并进行4参数曲线拟合(图 4)，结果见表2。使用EC50评估该测定方法的适用性，EC50值 应低于150 nM。对于两个读板机，EC 50值均低于85 nM，处 于可接受范围内。Cisbio将可接受的信噪比定义为≥. The microplate was then placed in a SpectraMax i3x reader and incubated at 37°C for the duration of the kinetic measurement. Modularity does provide some measure of forward vision into upcoming or future experimental needs. **On the SpectraMax iD3/iD5 readers only All graphs are for illustrative purposes only. Patented user-exchangeable cartridge design expands the system's detection capability making it. 제품. The SpectraMax i3x reader is engineered to perform with Spectral Fusion Illumination for increased sensitivity across the entire wavelength range, and a cooled photomultiplier tube (PMT) for improved detection in extremely. Order Now. The user guide also covers the features, specifications, and software of the reader, as well as the installation and. MD Neurotransmitter Transporter Assay. For each assay kit, a preconfigured protocol is included in SoftMax Pro Software to facilitate the acquisition and analysis of data for a simplified workflow. Every step is optimized for data acquired from a Molecular Devices microplate reader or data imported from. 0 | 9 reviews | Write your own review Measures Absorbance, Fluorescence, and Luminescence with the added functionality of modular upgradesMolecular Devices SpectraMax i3x Multi-Mode Microplate Platform. We would like to show you a description here but the site won’t allow us. ATP standard curves read on SpectraMax microplate readers. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a. 価格、見積もり、お近くの販売店を. It also integrates with the. Increased fluorescence performance. Fluorescence was measured using a microplate fluorometer (SpectraMax i3x, Molecular Devices, Beckman Coulter, CA, USA) with 365 nm excitation and 450 nm emission filters. Explore a wealth of applications with the future-ready SpectraMax i3x. 6% for the on-board optics (monochromators). Molecular Devices Software. SpectraMax Quant dsDNA Assay Kits. The SpectraMax i3 Multi-Mode Detection Platform is a versatile instrument that can perform various assays and measurements on microplates and cuvettes. The SpectraMax ® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of modular. Because a PMT needs to be set to a specified gain to reach a specific sensitivity,The SpectraMax® i3 Multi-Mode Detection Platform comes with standard spectral absorbance, fluorescence, and luminescence detection. 在SpectraMax i3x和SpectraMax iD5微孔板读板机上使用 SoftMax Pro软件绘制标准曲线，并进行4参数曲线拟合(图 4)，结果见表2。使用EC50评估该测定方法的适用性，EC50值 应低于150 nM。对于两个读板机，EC 50值均低于85 nM，处 于可接受范围内。Cisbio将可接受的信噪比定义为≥. 1. 13. com. Actual shake speed is based on the microplate format. for the SpectraMax M3/M4 and FlexStation® 3 Multi-Mode Microplate Readers. Concentrating the excitation light on the sample through label specific optics, high levels of sensitivity and performance are reached. This is not due to a temperature-dependent alteration in cellular autofluorescence,. KEY FEATURES • Combine cellular imaging and microplate assays into a workflow • Eliminate cell staining for cell counting and confluency with StainFree TechnologyWe would like to show you a description here but the site won’t allow us. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. One sensor measures the temperature of the top chamber heating foil, and another is used to measure the bottom chamber heating foil. Multiple automated tests are provided including: • Linearity • Precision • Accuracy • Dynamic range • Alignment • Signal to noise • Signal to background • Filter checks iD5. A SpectraMax® i3x was used to determine fluorescence with an excitation wavelength of λ = 360 nm and emission at λ = 460 nm. Using the SpectraMax i3x reader, the preconfigured SpectraMax Glo Steady-Luc Reporter assay protocol in SoftMax Pro Software was used to measure luminescence. CHO M1WT3 cells are Chinese hamster ovary cells stably transfected with the M1 muscarinic G q -coupled receptor. These concentrations are then used to perform western blots. The following detection cartridge is required: • Fluorescence Polarization (FP-FLUO) Detection Cartridge (Molecular Devices cat. SoftMax Pro. 08, 0. +44 118 944 8000. Fluorescence intensity was measured using a Spectramax i3x plate reader (Molecular Devices) with excitation at 575 nm (bandwidth 9 nm) and emission at 608 nm (bandwidth 15 nm). Spectral Fusion Illumination is a powerful combination of Xenon flash lamp and LEDs that provides unmatched signal strength*On the SpectraMax i3x reader only. SPECTRAmax 190 Microplate Spectrophotometer Operator™s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax 190 control panel are shown in boxed Helvetica type. The SpectraMax® i3x offers a wealth of applications; it can measure fluorescence, absorbance and luminescence, and its modular upgrades enable western blotting, imaging cytometry and injectors. . Page 9 Setting Up the SpectraMax® MiniMax™ 300 Imaging Cytometer 2. 2C). It allows you to explore cellular pathways and protein activation and expression in one system. 12 mg/mL) of polysaccharides were prepared after addition of glucosum anhydricum for calibration. 0200-7029 Wavelength range (LUM): Visible to 650 nm Dead volume: < 10 µL with Reverse . EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. Red and green fluorescence was then measured (emission at 590 and 530 nm, respectively, with excitation at 490 nm) using a fluorescence microplate reader (Spectra Max i3x, Molecular Devices Inc. The corrected absorbance is within 5% of the. Thetypeof luciferase(Renilla. Multimode Reader Comparison Chart. 0´ 104 8. Danaher Inc spectramax i3x. The SpectraMax® Paradigm® Multi-Mode Microplate Reader measures absorbance, fluorescence, time-resolved fluorescence (including HTRF), fluorescence polarization, AlphaScreen®, AlphaLISA®, and luminescence assays for up to 1536-well plates. Because a PMT needs to be set to a specified gain to reach a specific sensitivity,i3x Multi-Mode Microplate Reader. 6 in. With a preconfigured protocol to simplify data acquisition and analysis, SoftMax Pro Software completes this total solution for fast, sensitive, and reliable dual luciferase reporter assay results. 304 signal level saturation. The SpectraMax i3x multi-functional detection platform launched by Molecular Devices can meet the multi-functional detection system with unlimited possible applications as your future needs change. Find regional support. Explore a wealth of applications with the future-ready SpectraMax i3x. Instrument. SoftMax Pro Software was used to analyze data, and the net ΔRFU values calculated were used to plot a standard curve, generate the R 2 value, and show the sensitivity of this reader in detecting very low endotoxin levels. Contact Local Team. HTRF IP1 standard curve measured on the SpectraMax i3x (green circles) and SpectraMax iD5 (red circles) readers. 0112-0115J 9 Chapter1:Introduction TheSpectraMax®M3Multi-ModeMicroplateReader,SpectraMax®M4Multi-ModeMicroplate Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-ModeSpectraMax MiniMax 300 イメージングサイトメーター は、研究室でのアップグレードが可能なSpectraMax i3xシステム専用のオプションで、ユーザーは複雑なイメージングシステムに投資することなく、細胞増殖を解析できます。SPECTRAmax PLUS 384 Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS 384 control panel are shown in boxed Helvetica type. SpectraMax M2, M2e; SpectraMax M3, M4, M5, M5e; FilterMax™. iD5. The chemical reduction of resazurin was evaluated incubating each well of the microtiter plate with 10 μL (10% of final well volume) of 0. Synthesis of ZM-1NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. Bioz Stars score: 98/100, based on 1 PubMed citations. Molecular Devices’ ScanLater Western Blot Detection Cartridge uses TRF detection to turn its SpectraMax i3X and Paradigm plate readers into Western blot readers. Examples: fiPress carefully to engage,fl fiDo not press down. Afterwards, 25 μL SpectraMax Glo Steady-Luc working solution was added to the wells containing luciferase and controls. The calculated LOQ values were 0. ZERO BIAS - scores, article reviews, protocol conditions and moreWe would like to show you a description here but the site won’t allow us. Multiple automated tests are provided including: • Linearity • Precision • Accuracy • Dynamic range • Alignment • Signal to noise • Signal to background • Filter checks. SPECTRAmax 340PC Microplate Spectrophotometer Operator’s Manual 1-3 Introduction General Overview The SPECTRAmax ® 340PC incorporates a holographic grating monochromator which allows you to specify a precise wavelength, from 340 nm to 850 nm, for the absorbance maximum of your sample. KEY FEATURES • Combine cellular imaging and microplate assays into a workflow • Eliminate cell staining for cell counting and confluency with StainFree TechnologySpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers Operator’s Manual — 0112-0115 Rev. QBT Fatty Acid Transporter Assay. Afterwards, 25 μL SpectraMax Glo Steady-Luc working solution was added to the wells containing luciferase and controls. After correcting for negative controls and quench standards, the soil enzyme activities were expressed in units of nmol h −1 g −1 soil (German et al. Fluorescence Microplate Reader Comparison Chart. Microplate assays were performed on a multimode microplate detector (Molecular Devices Spectra Max i3x). 7°C (gradient heating design). It allows you to explore cellular pathways and protein activation and expression in one system. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. This document is copyright protected and any reproduction of this document, in whole or any part, is strictly prohibited, except as Molecular Devices may authorize in. Figure 3. Table 1. Absorbance: For wavelengths in the visible range, 340 nm and above, clear polystyrene plates are recommended. Adding time resolved fluorescence, HTRF® or western blot detection simply requires the user to exchange a detection module. Figure 1. 315 can't find zero order. 5577 Web: Email: [email protected] SpectraTest® Validation Plates are valuable tools for verifying that absorbance-, fluorescence-, and luminescence-capable microplate readers are operating correctly for GMP and GLP laboratories. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. 02, 0. Certified SpectraMax ® M5 e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays HTRF (Homogeneous Time-Resolved Fluorescence) technology. 305 reference level saturation. 4. Time-resolved fluorescence was measured on the SpectraMax Multi-Mode Microplate Readers (see Table 2 for instrument settings). Structured Review. Get a front row view of your cells. Measure oxidative metabolism and glycolytic activity on the SpectraMax i3x reader. Data from Analyst®, and FLIPR® systems can also be easily imported and analyzed. 312 gain calibration failed. Spectranet customer portalThe SpectraMax Paradigm Multi-Mode Detection Platform is a modular multi-mode microplate detector. SpectraMax® i3 Multi-ModeDetectionPlatform withtheSpectraMax® MiniMax™ImagingCytometer UserGuide 5025027A April2013. SpectraMax® i3x Multi-Mode Microplate Reader with MiniMax™ imaging cytometer Integrated multi-mode microplate reader and imaging system • Ideal for cell counting, label-free imaging, and cell proliferation • Western blot, imaging, and injectors on one configurable microplate reader • Stain free, automated cell countingusing the SpectraMax DuoLuc Reporter Assay Kit on the SpectraMax i3x microplate reader. Dependingontheapplication,theinstrumentcanread6,12,24,48,96,and384-well microplates. Page 9 Installing and Setting Up the SpectraMax MiniMax Imaging Cytometer Attaching the Imaging Cytometer to the Microplate Reader Attaching the SpectraMax MiniMax Imaging Cytometer to the SpectraMax i3x Instrument requires a work area that is approximately 130 cm (51. Also review the Maintenance and Troubleshooting chapter of the user guide for your model plate reader.